Total Petroleum Hydrocarbon ( TPH ) are environmental contaminants that are released into the marine environment through oil spills, industrial and domestic activities. TPH are readily transformed into more
hydrophilic metabolites, which are accumulated in bile. Thus fish bile can be used as a biomarker of exposure of fish to TPH in the marine environment. in this study several stations were selected from the Khure Mussa in the northern part of the Persian Gulf. Fish and sediment sampling were collected in the 2009. Preparation and analysis of the samples were performed according to MOOPAM method using Spectro florophotometer (UVF(. The highest average concentration of TPH in sediment was 364.91 mg/kg (dry weight) and was observed in Jafari station. The maximum concentration of TPH in bile liquid of fish (Euryglossa orientalis ) was 525 mg/l which was found in Zangi station. Statistical analysis revealed a significant differences between TPH concentration in sediment and in bileliquid of fish (Euryglossa orientalis ) in the study area (Pfish bile observed in this study suggests that fish bile can be used as a tool for bio-monitoring of TPH pollution.