S. Eskandari; H. Mozdarani; A. Mashinchian Moradi; M. H. Shahhosseiny
Volume 2, Issue 4 , December 2012, , Pages 215-224
Abstract
Crude oil is enriched in polycyclic aromatic hydrocarbons (PAHs). Many PAH analogs have proved to potentially damage DNA. DNA damage can be assessed using various biomarkers to find out the degree of genotoxicity of pollutants following in vitro exposure. In this research the comet assay and micronucleus ...
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Crude oil is enriched in polycyclic aromatic hydrocarbons (PAHs). Many PAH analogs have proved to potentially damage DNA. DNA damage can be assessed using various biomarkers to find out the degree of genotoxicity of pollutants following in vitro exposure. In this research the comet assay and micronucleus (MN) test were used to detect DNA damages and cytogenetic changes following crude oil exposure. For this purpose, freshwater bivalve mollusks (Anodonta cygnea) were exposed for ten days to 0.25, 0.5 and 1.0 ppm of crude oil. For the comet assay and for the MN test, hemolymph and gill cells of mussels were sampled respectively. Statistically, significant increase of DNA damage and micronuclei were found with 0.25, 0.5 and 1.0 ppm of crude oil. This study was performed to ascertain that A. cygnea is a good bioindicator of pollutants in aquatic environments; also identified hemolymph and gill of A.cygnea are most effective and practical tissues for genotoxicity studies.
S. Rahmani; P. Ghavam Mostafavi; M. H. Shahhosseiny; Gh. Vosoughi; A. Faraji
Volume 1, Issue 1 , December 2011, , Pages 43-50
Abstract
Coral reefs which form some of the most diverse ecosystems on Earth support many symbiotic relationships. Symbiodinium can provide up to 90% of a coral.s energy requirements. Temperature rise, turbid water and high salinity in the Persian Gulf were among the factors separating zooxanthellae from corals ...
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Coral reefs which form some of the most diverse ecosystems on Earth support many symbiotic relationships. Symbiodinium can provide up to 90% of a coral.s energy requirements. Temperature rise, turbid water and high salinity in the Persian Gulf were among the factors separating zooxanthellae from corals and result in bleaching phenomenon. Therefore, it is crucial to identify Symbiodinium of the Persian Gulf corals. Since zooxanthellae identification according to morphological characteristics is not precise enough, today, Symbiodinium different clades are identified using molecular techniques. In this study, Symbiodinium of genus Acropora as a dominant scleractinian coral in Farur Island, northern Persian Gulf were identified using molecular techniques. For this reason, sampling from depth of 7-10 meters was carried out. After DNA extraction, the target gene which was large subunit ribosomal RNA (28S rDNA) gene was amplified, using Polymerase Chain Reaction (PCR). Then the amplified fragments analyzed, by sequencing, and Symbiodinium clade was identified, using phylogenetic tree, and the situation of considered clade and its phylogenetic association with other studied samples in Iran and other parts of the world were defined. The
results showed that clade D was the only symbiont of Acropora off Farur Island. Since clade D is the most resistant clade against environmental changes, it is natural to identify this clade from Symbiodinium in Persian Gulf specific conditions.
P. Ghavam Mostafavi; Sh. Shahnavaz; M. Noroozi; M. R. Fatemi; M. H. Shahhosseiny; A. Mahvari
Volume 1, Issue 1 , December 2011, , Pages 69-73
Abstract
Nuclear markers such as microsatellites have allowed the identification of conservation and management populations of the Hawksbill turtles. In present study, eight microsatellite loci were studied. 60 samples of hawksbill turtles. flipper from Shidvar and Hormuz Islands have been surgically removed ...
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Nuclear markers such as microsatellites have allowed the identification of conservation and management populations of the Hawksbill turtles. In present study, eight microsatellite loci were studied. 60 samples of hawksbill turtles. flipper from Shidvar and Hormuz Islands have been surgically removed and preserved in 20% DMSO buffer. DNA was extracted using DNP KIT and amplified by PCR methods. The average number of alleles in Shidvar and Hormoz were 7 and 7.37 respectively with range of 7-13. The average expected and observed heterozygosity was 0.77 and 0.46 respectively. The linkage disequilibrium and deviations from Hardy-Weinberg equilibrium have been tested. The Fst values was 0.048, showing a significant difference between the two sites (P<0.01). The genetic distance between populations was found to be 0.27, which indicates that the genetic difference among the studied populations is pronounced. These results together with highly significant RST of genotypic differences between these pairs of samples support the existence of different genetic populations of Eretmochelys imbricata within the Iranian Islands of the Persian Gulf.