S. Eskandari; H. Mozdarani; A. Mashinchian Moradi; M. H. Shahhosseiny
Volume 2, Issue 4 , December 2012, , Pages 215-224
Abstract
Crude oil is enriched in polycyclic aromatic hydrocarbons (PAHs). Many PAH analogs have proved to potentially damage DNA. DNA damage can be assessed using various biomarkers to find out the degree of genotoxicity of pollutants following in vitro exposure. In this research the comet assay and micronucleus ...
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Crude oil is enriched in polycyclic aromatic hydrocarbons (PAHs). Many PAH analogs have proved to potentially damage DNA. DNA damage can be assessed using various biomarkers to find out the degree of genotoxicity of pollutants following in vitro exposure. In this research the comet assay and micronucleus (MN) test were used to detect DNA damages and cytogenetic changes following crude oil exposure. For this purpose, freshwater bivalve mollusks (Anodonta cygnea) were exposed for ten days to 0.25, 0.5 and 1.0 ppm of crude oil. For the comet assay and for the MN test, hemolymph and gill cells of mussels were sampled respectively. Statistically, significant increase of DNA damage and micronuclei were found with 0.25, 0.5 and 1.0 ppm of crude oil. This study was performed to ascertain that A. cygnea is a good bioindicator of pollutants in aquatic environments; also identified hemolymph and gill of A.cygnea are most effective and practical tissues for genotoxicity studies.
A. Mashinchian Moradi; M. AlidoustSalimi; M. H. Shahhosseini; P. AlidoustSalimi
Volume 2, Issue 4 , December 2012, , Pages 233-238
Abstract
Butachlor (N-(butoxymethyl)-2-chloro-N-2, 6-diethylacetanillide) is widely used chloroacetanilide herbicide in Asian region, especially in the north of Iran. Several studies have been reported on toxicity of butachlor, but little information is accessible on DNA damaging potential. Thus, the present ...
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Butachlor (N-(butoxymethyl)-2-chloro-N-2, 6-diethylacetanillide) is widely used chloroacetanilide herbicide in Asian region, especially in the north of Iran. Several studies have been reported on toxicity of butachlor, but little information is accessible on DNA damaging potential. Thus, the present study was undertaken to investigate of DNA damage by butachlor in Cyprinuscarpio(Pisces: Cyprinidae) using single cell gel electrophoresis. This study was done according to static method. The specimens were exposed to different concentrations of butachlor (0.07, 0.15 and 0.3 mgl-1) and sampling was carried out after 48 hour (short-time exposed). Based on the results, a significant increase in genetic damage index was observed in treatments compared with control samples (P<0.01). Also, the results showed the genotoxicity potential of this herbicide on fish. This study corroborated that comet assay is applied on the fish is a functional method to determine potential genotoxicity pollutants.
A. Mashinchian Moradi; H. Mozdarani; P. Alidoust Salimi; M. Alidoust Salimi
Volume 2, Issue 3 , August 2012, , Pages 185-188
Abstract
Malathion (S-(1, 2-dicarboethoxyethyl) O, O-dimethyl phosphorodithioate) is one of the organophosphate pesticides comprehensively used in agriculture fields throughout the world. In spite of widely used, little information are available about DNA damage in aquatic organisms. Therefore, the present study ...
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Malathion (S-(1, 2-dicarboethoxyethyl) O, O-dimethyl phosphorodithioate) is one of the organophosphate pesticides comprehensively used in agriculture fields throughout the world. In spite of widely used, little information are available about DNA damage in aquatic organisms. Therefore, the present study carried out to investigation of DNA damage induced by malathion in Cyprinus carpio (Pisces: Cyprinidae) using single cell gel electrophoresis. The condition of experiment was determined in static system. The specimens were exposed to different non-lethal concentrations (0.5, 1.5 and 3 mgL-1) of the malathion for 96 hour (short-time exposed). Our results showed, the specimens exposed to different concentrations of malathion exhibited significantly higher DNA damage in their blood cells than the control sample (P<0.05). This study confirmed that the comet assay is useful method in determining genotoxicity of pesticides. Fish can be used for biomonitoring of the genotoxic pollutants in aquatic environment. Furthermore, DNA strand breakage can be used as biomarker in ecotoxicological studies.
A. Mashinchian Moradi; A. H. Dashti; M. R. Fatemi; P. Aberoumand Azar
Volume 2, Issue 2 , June 2012, , Pages 171-176
Abstract
Tracking and determining the amount and types of urban sewage that is being releasing to the environment is important in planning for wastewater treatment systems and water quality control. In this research, in four stations of Anzali Lagoon, (by means of a gas chromatograph device with mass ...
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Tracking and determining the amount and types of urban sewage that is being releasing to the environment is important in planning for wastewater treatment systems and water quality control. In this research, in four stations of Anzali Lagoon, (by means of a gas chromatograph device with mass detector spectrometer) wastewater pollution has been measured and evaluated with molecular indicator Linear Alkyl Benzene(LABs) in bivalve “Anodonta cygnea”. Average concentrations of LABs were calculated 520 μg/kg dry weight in samples of station 1, 1245 in station 2, 2144 in station 3, and 1746 in station 4. Results from this study indicate severe LABs pollution in studied stations in Anzali Lagoon’s sugesing that urban wastewater has reached these stations.
F. Shariati; A. Esmaili Sari; A. Mashinchian Moradi; M. Pourkazemi
Volume 2, Issue 1 , March 2012, , Pages 81-90
Abstract
Tissue metal accumulations (gills, liver, kidney and muscle) in Persian sturgeon (Acipenser persicus) were compared folowing exposure to sublethal levels of waterborne Cd (50, 400 and 1000 μg•L-1) after periods of 1, 2, 4 and 14 days. The obtained results indicate that at the end of 4 and 14 ...
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Tissue metal accumulations (gills, liver, kidney and muscle) in Persian sturgeon (Acipenser persicus) were compared folowing exposure to sublethal levels of waterborne Cd (50, 400 and 1000 μg•L-1) after periods of 1, 2, 4 and 14 days. The obtained results indicate that at the end of 4 and 14 days of exposure, total tissue cadmium concentration followed the pattern: liver > gill > kidney > muscle. Calculation of bioconcentration factor (BCF) after 14 days exposure showed that at low and high concentrations, highest BCFs were found in kidney and liver, respectively. According to the results, the accumulation capacity of muscle was the lowest at all exposure concentrations. Cd concentration in the cytosol of experimental tissues were measured and the results indicated that Cd levels in the cytosol of liver, kidney and gills increased 240.71, 32.05, and 40.16-fold, respectively 14 days after exposure to 1000 μgL-1 Cd. The accumulation of Cd in cytosol of tissues is in the order of liver> gills> kidney.
A. Mashinchian Moradi; Sh. Zirehpour; M. B. Bahadori; Gh. Vossughi; M. B. Nabavi
Volume 2, Issue 1 , March 2012, , Pages 129-134
Abstract
Total Petroleum Hydrocarbon ( TPH ) are environmental contaminants that are released into the marine environment through oil spills, industrial and domestic activities. TPH are readily transformed into more hydrophilic metabolites, which are accumulated in bile. Thus fish bile can be used as a biomarker ...
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Total Petroleum Hydrocarbon ( TPH ) are environmental contaminants that are released into the marine environment through oil spills, industrial and domestic activities. TPH are readily transformed into more hydrophilic metabolites, which are accumulated in bile. Thus fish bile can be used as a biomarker of exposure of fish to TPH in the marine environment. in this study several stations were selected from the Khure Mussa in the northern part of the Persian Gulf. Fish and sediment sampling were collected in the 2009. Preparation and analysis of the samples were performed according to MOOPAM method using Spectro florophotometer (UVF(. The highest average concentration of TPH in sediment was 364.91 mg/kg (dry weight) and was observed in Jafari station. The maximum concentration of TPH in bile liquid of fish (Euryglossa orientalis ) was 525 mg/l which was found in Zangi station. Statistical analysis revealed a significant differences between TPH concentration in sediment and in bileliquid of fish (Euryglossa orientalis ) in the study area (Pfish bile observed in this study suggests that fish bile can be used as a tool for bio-monitoring of TPH pollution.
P. Aberoomand Azar; Sh. Moradi; Sh. Piramoon; A. Mashinchian Moradi
Volume 1, Issue 1 , December 2011, , Pages 23-28
Abstract
TiO2 photo catalyst was prepared in presence of nano SiO2 and Hydroxy Propyl Cellulose (HPC) by sol gel method with Titanium Tetra IsoproPoxide (TTIP) as titanium precursor. Additional effect of HPC in preparation of nano catalyst, and photo catalyst properties measured and characterized by FT-IR, FT-Raman ...
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TiO2 photo catalyst was prepared in presence of nano SiO2 and Hydroxy Propyl Cellulose (HPC) by sol gel method with Titanium Tetra IsoproPoxide (TTIP) as titanium precursor. Additional effect of HPC in preparation of nano catalyst, and photo catalyst properties measured and characterized by FT-IR, FT-Raman and SEM. The catalyst size and structural properties of the film were determined by .-Ray Diffraction (.RD). The nano particles of TiO2/SiO2 with HPC contained anatase phase in advance. The surface area measured by the Brunauer, Emmett and Teller (BET) method. Presences of Ti and Si in the nano structure were confirmed by Scanning Electron Microscopy (SEM) equipped with Energy Dispersive .-ray (ED.) spectroscopy. Photo catalyst properties of TiO2/SiO2 examined at first for decomposition of Methyl Red as azo dye, and then for degradation of 5ppm diazinon solution as an organo phosphorous insecticide pollutant in marine source, (Caspian Sea water). Concentration of diazinon during of degradation by TiO2/SiO2 photo catalyst was monitored by UV-Vis spectrophotometery. The insecticide degradation occurred within 105 min of photocatalytic treatment.
A. Mashinchian Moradi; M. Jalali Mosallam; M. R. Fatemi
Volume 1, Issue 1 , December 2011, , Pages 51-58
Abstract
In this study, the amount of nickel and vanadium elements in the soft tissues of rock oysters (Saccostrea
cuculata) and sea water from coastal areas of Qeshm Island, the biggest island of Persian Gulf located in the south of Iran, were measured to evaluate the contamination of the aquatic environment ...
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In this study, the amount of nickel and vanadium elements in the soft tissues of rock oysters (Saccostrea
cuculata) and sea water from coastal areas of Qeshm Island, the biggest island of Persian Gulf located in the south of Iran, were measured to evaluate the contamination of the aquatic environment and organisms. Following the bioassay, weighing and chemical digestion of the samples, finally the amount of nickel and vanadium in the soft tissues of the oysters and sea water samples were measured via the ICP system. The results indicated that the average concentration of nickel and vanadium found in the sea water were respectively: 15.7 and 37.9 micro grams per liter and in the soft tissues of oyster: 2.44 and 1.91 mg per kg respectively. In comparison with the FDA and WHO standards, only the concentration of nickel in the soft tissues of oysters was in a permitted level, and the rest exceeded the permitted levels. The average concentration of metals in the north side of the island were more than that found in south of the island but
this difference was not statistically significant (p> 0.05).